5′ DNA Adenylation Kit

5′ DNA  Adenylation Kit
产品描述

5’ DNA腺苷酸化试剂盒用于合成5’ DNA腺苷酸化单链DNA接头,可生成有3’终止子和无3’终止子的腺苷酸化DNA。该试剂盒可简单有效的将pDNA转化为AppDNA,转化率高达95%。
 
名称 Cat# 描述 价格 数量
5’ DNA Adenylation Kit
APPK-100
10 reactions,50 μM
640.00
5’ DNA Adenylation Kit
APPK-200
50 reactions,50 μM
2,400.00

制品说明:
5’ DNA腺苷酸化试剂盒用于合成5’ DNA腺苷酸化单链DNA接头,可生成有3’终止子和无3’终止子的腺苷酸化DNA。该试剂盒可简单有效的将pDNA转化为AppDNA,转化率高达95%。

产品特点:

  • 一步反应,简单且有效
  • 65℃降低二级结构
  • 高效,无需纯化产物
  • 模板量由pmol 到umol 单位级容易扩增

应用: 酶催化生成5’ DNA腺苷酸化单链DNA接头,用于NGS。

5' DNA Adenylation Kit

产品组分:
10X 5 DNA Adenylation Reaction Buffer
25 mM ATP
Mth RNA Ligase

10X 5 DNA Adenylation Reaction Buffer

500 mM NaAc (25℃条件下,pH 6.0)
100 mM MgCl2 
50 mM DTT 
1 mM EDTA

储存条件:-20℃保存

Manual & Protocols

There is no supporting data for this product. Will be available soon!!
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