HoTaq DNA聚合酶(热启动)

HoTaq DNA聚合酶(热启动)
产品描述

HoTaq DNA聚合酶是经过修改的热启动Taq DNA聚合酶。HoTaq DNA聚合酶处于未 激活状态,常温下酶的活性极低,低温条件下能 够 抑制引物二聚体的形成及引物的非特异性退火 引起的非特异性扩增,具有很高的特异性。可用于无小条带的诊断反应。
 
名称 Cat# 大小 浓度 价格 数量
HoTaq DNA 聚合酶(热启动)
HT-200
500 units
5 U/μl
1,050.00
HoTaq DNA 聚合酶(热启动)
HT-205
2,500 units
5 U/μl
4,200.00
HoTaq DNA 聚合酶(热启动)
HT-210
5,000 units
5 U/μl
7,770.00
HoTaq DNA 聚合酶(热启动)
HT-OEM
Any Size
询价

制品说明:
HoTaq DNA聚合酶是经过修改的热启动Taq DNA聚合酶。HoTaq DNA聚合酶处于未 激活状态,常温下酶的活性极低,低温条件下能 够 抑制引物二聚体的形成及引物的非特异性退火 引起的非特异性扩增,具有很高的特异性。可用于无小条带的诊断反应。该 酶具有高持续性5'-> 3' DNA 聚合酶活性,无3'-> 5'核酸外切酶活性。每一批HoTaq DNA聚合酶均经过PCR扩增检测。
本产品是经过化学修饰的Taq聚合酶。室温下为未激活状态,94℃条件下10分钟可激活本酶。

同时提供:
10x PCR Buffer I (default)
Or 10x PCR Buffer II (with dNTP)
(请在订货时注明)

10X PCR Buffer I:
100mM Tris-HCL
500mM KCl
15mM MgCl2
pH 8.3@25 oC

来源:
E.coli菌株,携带来自Thermus aquaticus 的Taq DNA聚合酶基因。

用途:
·DNA扩增
·ssDNA和dsDNA测序
·基因定点 突变

比较:
以下是MC L AB的HoTaq与其他 著名品牌产品的比较

推荐贮存条件:
-20℃

推荐反应条件:
94°C, 10min. -> (94°C, 10sec. -> 55°C, 30sec. ->72°C, 30sec.) for 25 cycles.

单位定义:
1单位是指75℃条件下,30分钟 ,能将10 nmol dNTPs掺入酸性不溶物所需要的酶的量。

参考文献:
1.         Chien A, Edgar DB, Trela JM (1976). "Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus". J. Bact. 127 (3): 1550–7. PMC 232952. PMID 8432.
2.         Saiki, RK; et al. (1988). "Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.". Science 239 (4839): 487–91. doi:10.1126/science.2448875. PMID 2448875.
3.         Saiki, RK; et al. (1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". Science 230 (4732): 1350–4. doi:10.1126/science.2999980. PMID 2999980.
4.         Lawyer FC, et al. (1993). "High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase ...". PCR Methods Appl. 2 (4): 275–87. PMID 8324500.



COA ( Certificate of Analysis )



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