Dh5-Alpha Competent E. coli

¥1,308.62¥41,637.75

DH5a™ is the most frequently used E. coli strain for routine cloning applications.
SKU: DA 分类: ,
SKU OPTIONS 价格
DA-100 20x50ul (20 tubes), >1x10⁹ ¥1,308.62
DA-196 96x50ul (96-tube rack), >1x10⁹ ¥3,331.02
DA-144A* Pre-payment for one year, 144x10x100μl or 15 racks of DA-196 Kit, individual shipping and handling charges will apply. >1x10⁹ ¥41,637.75

描述

Description:
DH5a™ is the most frequently used E. coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Application:

  • Highest transformation efficiency
  • General cloning
  • Blue-white selection
  • Plasmid isolation

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Genotype:
F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1

Reference:
Woodcock, D.M., Crowther, D.M., Doherty, J., Jefferson,S., DeCruz, E., Noyer-Weidner, M., Smith, S.S., Michael, M.Z., and Graham, M.W., Nucl. Acids Res. (1989) 17, 3469-3478
Competent Cell Type: Chemically

Transformation Efficiency (cfu/μg):
>1×109

Blue-Whiet Screening:
Yes

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
No

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

RecA Deficient:
Yes

其他信息

OPTIONS

20x50ul (20 tubes), >1×10⁹, 96x50ul (96-tube rack), >1×10⁹, Pre-payment for one year, 144x10x100μl or 15 racks of DA-196 Kit, individual shipping and handling charges will apply. >1×10⁹

Manual & Protocols

COA ( Certificate of Analysis )

The Q&A for this product will be available soon.