Recombinant Human Wnt-5a Protein

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The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation.

SKU: SKU49574 分类: ,
SKU OPTIONS 价格
WNT5a-100 50 ug (1 mg/ml) Please inquire
WNT5a-200 1 mg (1 mg/ml) Please inquire
WNT5a-300 100 mg (10 mg/ml) Please inquire
WNT5a-400 1 g (10 mg/ml) Please inquire
WNT5a-OEM Any size Please inquire

描述

Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Wnt 5a belongs to the non-canonical class of Wnt proteins which are independent of or inhibit β-catenin signalling. Wnt5a when bound to its receptor triggers an intracellular Ca release which activates PKC and CaMK11 which in turn activates TAK1 MAPkinase-kinase and nemo-like (NLK) kinase. NLK phosphorylates Lef1/TCF protein complex inhibiting the formation of the β-catenin Lef1/TCF DNA complex.
 
Full Name
Wingless-type MMTV Integration Site Family Member 5a

 Source
E. coli

Species
Human

Accession #
P22725

Molecule Weight
The mature recombinant human Wnt-5a is a 38 kDa protein containing 343aa (Gln38-Lys380).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.

Storage
-80     Avoid repeated freeze-thaw cycles.

Storage Buffer
PBS

其他信息

OPTIONS

50 ug (1 mg/ml), 1 mg (1 mg/ml), 100 mg (10 mg/ml), 1 g (10 mg/ml), Any size

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