外切酶抗性随机引物

Dye 终止剂 5X测序缓冲液
Placeholder Fragment Analysis Matrix

¥0.00 – ¥5,948.00

保护引物免受3′→ 5′核酸外切酶降解并保证在DNA合成过程中充当有效引物。仅限研究使用。
SKU: ERRP Categories: ,
SKU OPTIONS 价格
ERRP-100 100µl, 100 reactions, 500 µM (1.1 µg/µl) ¥238.00
ERRP-110 1,000µl , 1,000 reactions, 500 µM (1.1 µg/µl) ¥1,190.00
ERRP-120 10,000µl, 10,000 reactions, 500 µM (1.1 µg/µl) ¥5,948.00
ERRP-OEM Any size, 500 µM (1.1 µg/µl) Please inquire

Description

制品说明：
MCLAB外切酶抗性随机引物为单链随机寡聚核苷酸混合物。该产品充当多种DNA合成反应的高效随机引物。本产品中的引物有两个硫化磷酸（PTO）修饰的3′-末端，使得其对校对DNA聚合酶，比如Klenow 大片段和phi29 DNA聚合酶，的3′→ 5′外切酶活性产生抗性 (1)。该引物还有5′-和 3′-羟基末端。本产品为即用型、20X浓缩水溶液。

应用：

  • 基因组DNA（2）、质粒DNA和噬菌体DNA(3)的链置换扩增
  • 随机引物DNA标记 (4-6).

浓度：
500 µM (1.1 µg/µl)

质量控制：
使用phi29DNA聚合酶对DNA合成过程中的高效引物进行功能测试。

储存条件： 4℃

参考文献：
1. Skerra, A., Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity, Nucleic Acids Res., 20, 3551-3554, 1992.
2. Dean, F.B., et al., Comprehensive human genome amplification using multiple displacement amplification, Proc. Natl. Acad. Sci., 99, 5261-5266, 2002.
3. Dean, F.B., et al., Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification, Genome Res., 11, 1095-1099, 2001.
4. Feinberg, A.P. and Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem., 132, 6-13, 1983.
5. Feinberg, A.P. and Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Addendum, Anal. Biochem., 137, 266-267, 1984.
6. Mackey, J., et al., Use of random primer extension for concurrent amplification and nonradioactive labeling of nucleic acids, Anal. Biochem., 212, 428-435, 1993.

Additional information

OPTIONS

100µl, 100 reactions, 500 µM (1.1 µg/µl), 1,000µl , 1,000 reactions, 500 µM (1.1 µg/µl), 10,000µl, 10,000 reactions, 500 µM (1.1 µg/µl), Any size, 500 µM (1.1 µg/µl)

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