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TAE (Tris/Acetate/EDTA) Buffer, 10X

Placeholder Sodium Hydroxide
Placeholder Tris-Buffered Saline (TBS), 10X

¥0.00 – ¥975.56

TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.

SKU: TAE10 分类： ,
SKU OPTIONS 价格
TAE10-100 1 L ¥356.90
TAE10-200 4 L ¥975.56
TAE10-OEM OEM Please inquire

描述

Description:
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer.

Application:
Casting and running buffer for agarose gel electrophoresis of nucleic acids.

Notes:
0.4M Tris base, 0.4M acetate, 10mM EDTA, pH 8.0.

Recommended Storage Condition: Room temperature

Reference:
1. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989) 23, 6.7

其他信息

OPTIONS

1 L, 4 L, OEM

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