Direct DNA Solution

¥1,427.58¥5,734.16

Direct DNA Solution is for purifying genomic DNA from a variety of sample types, such as whole blood, tissues, bone marrow, buffy coat, body fluids, cultured cells, cell suspensions, and Gram-negative bacteria.

SKU OPTIONS 价格
GDES-100 100ml ¥1,428.00
GDES-200 500ml ¥5,734.00

描述

Description:
Direct DNA Solution is for purifying genomic DNA from a variety of sample types, such as whole blood, tissues, bone marrow, buffy coat, body fluids, cultured cells, cell suspensions, and Gram-negative bacteria. This method employs a unique purification matrix and reagents for lysing cells and capturing DNA. It is well-suited for standard PCR screening applications. There is no need to pre-lyse the sample or precipitate the DNA, and the purified DNA is ready to use in standard PCR.

Direct DNA Solution Protocol
1.Take out the whole sample or part of it for DNA extraction. Wash it if necessary.
2.Mechanically pulverize the sample into a mush. For example, slice or grind the sample.
3.Transfer pulverized sample to a sterile tube and mix well with 100uL (or other volume that can immerse the sample) of Direct DNA Solution.
4.Incubate overnight at 37C in an incubator.
5.Spin the tube at maximum speed for 10 min.
6.Use 1uL of the supernatant as template in PCR reaction (25uL) with compatible PCR master mix, for example, 2X BlueStar™ PCR Master Mix.

其他信息

OPTIONS

100ml, 500ml

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