DNA Polymerase I

¥0.00¥4,782.44

DNA Polymerase I is a DNA-dependent DNA polymerase. The enzyme catalyzes 5′ -> 3′ synthesis of DNA and exhibits 3′ -> 5’ exonuclease proofreading activity.
SKU: DPI 分类: ,
SKU OPTIONS 价格
DPI-100 2,500 units (10,000 U/ml) ¥1,594.00
DPI-200 10,000 units (10,000 U/ml) ¥4,782.00
DPI-OEM Any Size Please inquire

描述

Description:
DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5′ -> 3′ DNA synthesis activity in addition to both 3′ -> 5′ and 5′ -> 3′ exonuclease activities. The combination of DNA synthesis and 5′ -> 3′ nuclease characteristics enable nick-translation during DNA synthesis.

Applications:

  • Nick translation of DNA to obtain probes with a high specific activity
  • Second strand synthesis of cDNA

Source:
A recombinant E. coli strain carrying the PolA gene

Specific Activity: 6,850U/mg

Supplied in:
25 mM Tris-HCl
0.1 mM EDTA
1.0 mM DTTl
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x Blue Buffer

10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.

Recommended Storage Condition: -20°C

其他信息

OPTIONS

2,500 units (10,000 U/ml), 10,000 units (10,000 U/ml), Any Size

Manual & Protocols

 

COA ( Certificate of Analysis )

 

MSDS & Certificates

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