Taq DNA Ligase

¥0.00¥4,853.73

Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor.
SKU: TDL 分类: ,
SKU OPTIONS 价格
TDL-100 10,000 units (40,000 U/ml) ¥1,617.95
TDL-200 40,000 units (40,000 U/ml) ¥4,853.73
TDL-OEM Any Size Please inquire

描述

Description:
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor.

Figure:

Figure. Comparing to another vendor’s product, the activity of MCLAB’s Taq DNA ligase was tested with BstEII-digested Lamda DNA under 45°C for 15 minutes.

Applications:

  • Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction
  • Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification

Source:
A recombinant E. coli strain carrying the cloned Taq DNA Ligase gene

Supplied in:
10 mM Tris-HCl
50 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
0.1% TWEEN 20
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x Taq DNA Ligase Buffer

10x Taq DNA Ligase Buffer:
200 mM Tris-HCl
250 mM Potassium Acetate
100 mM Magnesium Acetate
10 mM NAD 1
100 mM DTT
0.1% Triton® X-100
pH 7.6 @ 25°C
Unit Definition:

One unit is defined as the amount of enzyme required to give 50% ligation of the 12-base pair cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 minutes at 45°C.

Specific Activity: 400 Unit/µg

Recommended Storage Condition: -20°C

其他信息

OPTIONS

10,000 units (40,000 U/ml), 40,000 units (40,000 U/ml), Any Size

Manual & Protocols

COA ( Certificate of Analysis )

MSDS & Certificates

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