Cas13a (CRISPR Associated Protein 13a)

¥0.00¥11,434.50

Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic
repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.

SKU: SKU49675 分类: , , ,
SKU OPTIONS 价格
CAS13a-100 Cas13a (100 μg,1 mg/ml) ¥2,298.45
CAS13a-200 Cas13a ( 1 mg,1 mg/ml) ¥11,434.50
CAS13a-300 Cas13a (100 mg,10 mg/ml) Please inquire
CAS13BUF-100 Cas13 Buffer (10X Cas13 Reaction Buffer,200 ul) ¥157.50
CAS13BUF-200 Cas13 Buffer (10X Cas13 Reaction Buffer,1 ml) ¥472.50
CAS13-Storage-100 Cas13 Storage Buffer (10X Cas13 Storage Buffer,200 ul) ¥157.50
CAS13-Storage-200 Cas13 Storage Buffer (10X Cas13 Storage Buffer,1 ml) ¥472.50

描述

Description

Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system. Cas13a functions similarly to Cas9, using a ~64-nt guide RNA to encode target specificity. The Cas13a protein complexes with the guide RNA via recognition of a short hairpin in the crRNA, and target specificity is encoded by a 28 – 30-nt spacer that is complementary to the target region. Cas13a cuts within the crRNA direct repeat in a pre-crRNA array to produce a mature Cas13a-crRNA complex.

In addition to programmable RNase activity, all Cas13s exhibit collateral activity after recognition and cleavage of a target transcript, leading to non-specific degradation of any nearby transcripts regardless of complementarity to the spacer. The RNA-activated RNA cleavage activity of Cas13a allows for RNA detection and potential diagnostic applications.

Applications
• Targeted RNA knockdown
• RNA editing
• Nucleic acid detection

Source
E. coli

Organism
Leptotrichia wadeii (strain F0279)

UniProt #
U2PSH1

Molecule Weight
The mature recombinant Cas13a is a 143.7 kDa protein containing 1182aa.

Purity
> 95 % as determined by SDS-PAGE

Storage
-20 Avoid repeated freeze-thaw cycles.

Cas13 Storage Buffer
0.3 M NaCl
20 mM Tris-HCl, pH 8.0
0.5 mM EDTA
1 mM DTT
50% Glycerol

10X Cas13 Reaction Buffer
200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5

10X Cas13 sherlock reaction buffer (requested)
200 mM HEPES, 90 mM MgCl2, pH 6.8

 

Citations & Technical Literature

1. Nature, Vol. 613, pages 588 – 594 (2023) / Read Article
Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA
Oleg Dmytrenko, et al.
LwCas13a was purchased from MCLAB Molecular Cloning Laboratories (Cas13a-100). Reactions (10 μl) containing 250 nM of LwCas13a and 500 nM of its cognate crRNA in the provided 1× Cas9 buffer”

2. Microbiology Spectrum, Vol. 10, No. 5 (2022)/ Read Article
Reverse Transcription-Loop-Mediated Isothermal Amplification CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2

Concha Ortiz-Cartagena, et al.
“0.15 μL Cas13a endonuclease (25 nM; MCLAB), 0.5 μL crRNA (50 nM; IDT), 2 μL reporter (1,000 nM; IDT), and 5 μL of a cDNA sample, adjusted to a final volume of 20 μL with dH2O”

3. Frontiers in Cellular and Infection Microbiology, Vol. 11 (2021)/ Read Article
A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection
Iqbal Azmi, et al.
“SHERLOCK assay of the above reaction mix was performed in a separate 1.5 ml Eppendorf tube which contains 1 μL of the Cas13a either from extracted pool or commercially (MCLAB, USA, Cat no. CAS13a-200) obtained source (at 63.3 μg/ml concentration),”

 

其他信息

OPTIONS

Cas13a (100 μg,1 mg/ml), Cas13a (
1 mg,1 mg/ml), Cas13a (100 mg,10 mg/ml), Cas13 Buffer (10X Cas13 Reaction Buffer,200 ul), Cas13 Buffer (10X Cas13 Reaction Buffer,1 ml), Cas13 Storage Buffer (10X Cas13 Storage Buffer,200 ul), Cas13 Storage Buffer (10X Cas13 Storage Buffer,1 ml)

COA ( Certificate of Analysis )

MSDS & Certificates

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