dCas13a
¥0.00 – ¥11,434.00
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic
repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.
| SKU | OPTIONS | 价格 | |
|---|---|---|---|
| dCAS13a-100 | dCas13a (100 μg,1 mg/ml) | ¥2,298.00 | |
| dCAS13a-200 | dCas13a (1 mg,1 mg/m) | ¥11,434.00 | |
| dCAS13a-300 | dCas13a (100 mg,10 mg/ml) | Please inquire | |
| Bio-dCas13a-100 | Biotinylated dCas13a (100 μg,1 mg/ml) | ¥2,298.00 |
- Description
- Additional information
- Documents
Description
Description
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system. Cas13a functions similarly to Cas9, using a ~64-nt guide RNA to encode target specificity. The Cas13a protein complexes with the guide RNA via recognition of a short hairpin in the crRNA, and target specificity is encoded by a 28 – 30-nt spacer that is complementary to the target region. Cas13a cuts within the crRNA direct repeat in a pre-crRNA array to produce a mature Cas13a-crRNA complex.
The dcas13a is a mutant cas13a. It can bind target RNA and can not cut the RNA.
Biotinylated dCas13a is N-terminal tagged with biotin for streptavidin-based experiments.
Applications
• Targeted RNA knockdown
• RNA editing
• Nucleic acid detection
Source
E. coli
Organism
Leptotrichia wadeii (strain F0279)
UniProt #
U2PSH1
Molecule Weight
The mature recombinant Cas13a is a 143.7 kDa protein containing 1182aa.
Purity
> 95 % as determined by SDS-PAGE
Storage
-20 Avoid repeated freeze-thaw cycles.
Cas13 Storage Buffer (Click to Order)
0.3 M NaCl
20 mM Tris-HCl, pH 8.0
0.5 mM EDTA
1 mM DTT
50% Glycerol
10X Cas13 Reaction Buffer (Click to Order)
200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5
10X Cas13 sherlock reaction buffer (requested)
200 mM HEPES, 90 mM MgCl2, pH 6.8
Additional information
| OPTIONS | dCas13a (100 μg,1 mg/ml), dCas13a (1 mg,1 mg/m), dCas13a (100 mg,10 mg/ml), Biotinylated dCas13a (100 μg,1 mg/ml), Cas13 Buffer (10X Cas13 Reaction Buffer,200 ul), Cas13 Buffer (10X Cas13 Reaction Buffer,1 ml), Cas13 Storage Buffer (10X Cas13 Storage Buffer,200 ul), Cas13 Storage Buffer (10X Cas13 Storage Buffer,1 ml) |
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