10 bp DNA Ladder

描述

Description:
The 10 bp DNA Ladder consists of nine 10-bp repeats and is suitable for sizing double-stranded DNA fragments from 20 bp to 140 bp. The 60 and 100-bp bands are invisible to serve as reference points. The double-stranded ladder can be visualized on 2% to 5% agarose gels after ethidium bromide staining and 4 – 20% TBE gels provide higher resolution.

This ladder is premixed with Gel Loading Dye, Blue (6X) for direct gel electrophoresis.

Figure.  10 bp DNA Ladder visualized by ethidium bromide staining on a 4 – 20% TBE agarose gel. Mass value is 0.4 μg/lane.

Application:
Determination of the molecular weight of nucleic acids for analyzing restriction digests and PCR products.
The 10 bp DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.

Effective Size Range:
20bp to 140bp

Concentration and Usage:
0.2 μg/μl, recommended loading amount is 2 μl per lane diluted with sample buffer. Incorporate ethidium bromide (0.5 μg/ml) into both the gel and running buffer to examine the gel directly under UV-illumination and avoid diffusion of the bands.

Storage Buffer:
10 mM Tris-HCl (pH 7.5)
1 mM EDTA

Stability:
This product is stable under room temperature for at least 1 month and at 4°C for at least 3 months.

Recommended Storage Condition:-20°C

其他信息

Manual & Protocols