Ab Cloning 5′-RACE Kit

¥0.00¥7,720.86

The Ab cloning RACE kit provides a novel method for performing both 5′ and 3’ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning both heavy and light chains of human and mouse antibodies.

SKU OPTIONS 价格
ABC5-100 10 rxn ¥5,104.00
ABC5-200 20 rxn ¥7,721.00
ABC5-OEM any size Please inquire

描述

General Description
The Ab cloning RACE kit provides a novel method for performing both 5′ and 3′ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning both heavy and light chains of human and mouse antibodies, respectively. MCLAB’s SmartRT reverse transcriptase is an engineered MMLV RT with increased thermostability, improved cDNA synthesis ability; and a reduced RNase H activity. The enzyme also has a terminal transferase activity.  This tailing activity (terminal transferase activity) of the SmartRT reverse transcriptase allows to synthesize complete cDNA by SMART (Switching Mechanism At 5′ end of RNA Transcript) cDNA synthesis technology. With 3′-5′ extra nuclear acids added to the 3′ end of the first-strand cDNA, 5′ SMART universal oligo contains terminal complementation to nuclear acids at 3′ end of the first-strand cDNA can be annealed to first-strand cDNA tail and serves as an extended template for RT. The switch of template from mRNA to 5′ SMART universal oligo produces a complete cDNA copy of transcript RNA with 5′ SMART universal oligo at the end. The cDNA transcript from smarter RT can be used for directly in 5′ and 3′ race with MCLAB I-5™ 2X High-Fidelity Master Mix. Our 5’/3′ Race kit is optimized for antibody cloning. It also can be used for any gene of interest.

Features

  • Specific full length cDNA enrichment technique
  • Optimized primer set for full length antibody sequencing
  • Simplified protocol without 5′ end adaptor-ligation step

Application
Antibody full length sequencing

Components

Components Cap Color Volume (10 rxn) Volume (20 rxn)
Oligo dT Primer Orange 10 µl 20 µl
5X RT Reaction Buffer Green 40 µl 80 µl
dNTP Yellow 10 µl 20 µl
DTT Yellow 5 µl 10 µl
SmartRT™ reverse transcriptase Clear 20 µl 40 µl
5′ Universal Primer Red 10 µl 20 µl
3′ IgG Primer Red 10 µl 20 µl
3′ IgM Primer Red 10 µl 20 µl
3′ IgE Primer Red 10 µl 20 µl
3′ Kappa Chain Primer Red 10 µl 20 µl
3′ Lambda Chain Primer Red 10 µl 20 µl
I-5™ 2X High-Fidelity Master Mix Purple 100 µl 200 µl
RNAse Inhibitor Blue 5 µl 10 µl
Storage Condition for above components: -20 °C
Cap Finder (Store at -80 °C) Clear 10 µl 20 µl

 

Recommended Storage Condition
-20°C

Shipping Condition
dry ice

其他信息

OPTIONS

10 rxn, 20 rxn, any size

 

MSDS & Certificates

RNAse Inhibitor
Figure 1.The RACE ready cDNA was synthesized from 1ug of total RNA from hybridoma cells using the Ab cloning RACE kit. 4 ul of cDNA were used for 5’ and 3’ RACE PCR. The gel image shows the amplified antibody heavy chain and light chain cDNA.