Endonuclease V, E. coli
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Endonuclease V, (Endo V) is a 3′-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.
| SKU | OPTIONS | 价格 | |
|---|---|---|---|
| ENV-100 | 50 ug (1 mg/ml) | Please inquire | |
| ENV-200 | 1 mg (1 mg/ml) | Please inquire | |
| ENV-300 | 100 mg (10 mg/ml) | Please inquire | |
| ENV-400 | 1 g (10 mg/ml) | Please inquire | |
| ENV-OEM | Any size | Please inquire |
- 描述
- 其他信息
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描述
Description
Endonuclease V, (Endo V) is a 3′-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.
Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3′ to a lesion.
Applications
• High-throughput methods for mutation research
• Studies in mutagenesis and DNA repair
• Mismatch cleavage
• Genotyping
Source
E. coli
Purity
> 95 % as determined by SDS-PAGE
Storage
-80 Avoid repeated freeze-thaw cycles.
Unit Definition
One unit of T4 Endonuclease V converts 1 µg of UV-irradiated plasmid DNA from covalently closed circles to nicked form in 30 minutes at 37°C.
其他信息
| OPTIONS | 50 ug (1 mg/ml), 1 mg (1 mg/ml), 100 mg (10 mg/ml), 1 g (10 mg/ml), Any size |
|---|
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