Klenow Fragment (3´-> 5´ exo-)


Klenow Fragment, 3′->5′ exo–, is the large fragment of E.coli DNA polymerase I with a molecular weight of 68kDa.

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KPIM-100 10,000 units (50,000 U/ml) ¥1,487.00
KPIM-200 50,000 units (50,000 U/ml) ¥4,759.00
KPIM-OEM Any Size Please inquire


Klenow Fragment, 3′->5′ exo–, is the Large Fragment of E.coli DNA Polymerase I with a molecular weight of 68kDa. It exhibits 5′->3′ polymerase activity, but lacks the 3′->5′ exonuclease activities. The 3′->5′ exonuclease activity of the enzyme is eliminated by mutations in the 3′->5′ exonuclease active site.

Specific Activity:


  • Random priming labeling
  • DNA sequencing by the Sanger dideoxy method
  • Second strand cDNA synthesis
  • Second strand synthesis in mutagenesis protocols

Supplied in:
20 mM Tris-HCl
100 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Supplied with: 10x Blue Buffer

10x Blue Buffer:
500mM NaCl
100mM Tris-HCl
100mM MgCl2
10mM DTT
pH 7.9 @ 25 ºC

Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C.

Recommended Storage Condition: -20°C



10,000 units (50,000 U/ml), 50,000 units (50,000 U/ml), Any Size

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