描述
Description:
Klenow Fragment, 3′->5′ exo–, is the Large Fragment of E.coli DNA Polymerase I with a molecular weight of 68kDa. It exhibits 5′->3′ polymerase activity, but lacks the 3′->5′ exonuclease activities. The 3′->5′ exonuclease activity of the enzyme is eliminated by mutations in the 3′->5′ exonuclease active site.
Specific Activity:
5,000U/mg
Applications:
- Random priming labeling
- DNA sequencing by the Sanger dideoxy method
- Second strand cDNA synthesis
- Second strand synthesis in mutagenesis protocols
Supplied in:
20 mM Tris-HCl
100 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C
Supplied with: 10x Blue Buffer
10x Blue Buffer:
500mM NaCl
100mM Tris-HCl
100mM MgCl2
10mM DTT
pH 7.9 @ 25 ºC
Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C.
Recommended Storage Condition: -20°C
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