Dh10-Beta E. coli 感受态细胞

描述

Description:
The DH10B competent E. coli has a transformation efficiency of 1 x 10⁹ cfu/µg for supercoiled DNA and is ideal for high-efficiency cloning and plasmid propagation. It enables stable replications of high-copy number plasmids. In comparison with DH5a, methylated DNA from genomic preparation could be efficiently transformed into DH10B, therefor DH10B cells are more applicable than DH5a in generating genomic libraries containing methylated cytosine or adenine residues.

Applications:

• Highest transformation efficiency
• General cloning
• Blue-white selection
• Reduced recombination of cloned DNA (recA1)

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ–

 Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1×10⁹

Blue-Whiet Screening:
Yes

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
Yes

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

RecA Deficien:
Yes

其他信息

Manual & Protocols