| SKU | OPTIONS | 价格 | |
|---|---|---|---|
| HIND-100 | 50,000 units, 200 units/ul | ¥1,416.00 | |
| HIND-200 | 50,000 units, 1,000 units/ul | ¥1,416.00 | |
| HIND-300 | 200,000 units, 200 units/ul | ¥4,247.00 | |
| HIND-400 | 200,000 units, 1,000 units/ul | ¥4,247.00 | |
| HIND-OEM | Any Size | Please inquire |
- Description
- Additional information
- Documents
Description
Recognition site: A^AGCTT
Source:
Purified from a strain of E. coli that bears the cloned Hind III gene.
Storage Buffer:
10 mM Tris-HCl (pH 7.4)
200 mM NaCl
0.5 mM EDTA
1mM DTT
0.5 mg/ml BSA
50% (v/v) Glycerol
10x Hind III Buffer
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
100 μg/ml BSA
pH 7.9 @ 25°C
Reaction Conditions:
Buffer at 37°C
Typical Ligation/Recut Assay Results:
Hind III-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Hind III.
Recommended Storage Condition: -20°C
Comments:
Does not cleave DNA when either the 5’A or the C residue is N6 –methyladenine or 5-methylcytosine, respectively. Resistant to heat inactivation (10 minutes, 65°C).
Additional information
| OPTIONS | 50,000 units, 200 units/ul, 50,000 units, 1,000 units/ul, 200,000 units, 200 units/ul, 200,000 units, 1,000 units/ul, Any Size |
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