MutS Protein (Thermus aquaticus)

描述

Description
The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This Muts protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Taq MutS is active at temperature between 0 to 75°C. Since Taq MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels or on a solid phase such as Ni agarose or beads or magnetic Ni-NTA particles.

Applications
• Remove mismatch DNA (error correction) from gene synthesis reaction
• Mutation detection and removal
• Rapid isothermal SNP detection

Full name
Thermus aquaticus MutS DNA mismatch repair protein

Source
E. coli

Fusion Tag
6XHis tag at C-terminus

Accession #
AAC43637

Molecule Weight
92.8 kDa

Purity
> 98 % as determined by SDS-PAGE

Storage Buffer
20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1mM EDTA, 1 mM DTT, 50% Glycerol

Reaction Buffer
100mM KCl, 50 mM Tris-HCl, pH 8.5, 10 mM MgCl₂, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C

Storage
-20°C Avoid repeated freeze-thaw cycles.

References

1. Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 2004 Nov 23;32(20):e162.

2. Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 2005 Mar 30;33(6):e55.

3. MutS as a tool for mutation detection. Acta Biochim Pol. 2005;52(3):575-83. Epub 2005 Aug 4.

4. One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36.

5. Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods – 4, 257 – 262 (2007) doi:10.1038/nmeth1007

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