PcrA 螺旋酶

¥2,289.00

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PcrA -100 PcrA Helicase (100 μg, 0.5 mg/ml) ¥2,289.00

Description

Description
PcrA is an ATP-driven 3′ to 5′ helicase responsible for the unwinding of double-stranded DNA (dsDNA). PcrA binds to and unravels of dsDNA into two strands of single-stranded DNA (ssDNA).

Applications Helicase-dependent DNA isothermal amplification

Source E. coli

Organism Geobacillus stearothermophilus

Storage -20°C

Storage Buffer
600 mM NaCl, 50 mM Tris pH 7.6, 50% glycerol

10X Reaction Buffer
500 mM Potassium acetate, 200 mM Tris-acetate, 100 mM Magnesium acetate, 1 mg/ml BSA, pH 7.9

Experiment Result

Helicase-dependent DNA isothermal amplification reaction (65 °C, 1 hour) using PcrA helicase for cloning 667 bp and 871 bp DNA fragments. Template of the reaction is 5 ng DNA.

References
1. M. Dittrich, et al., (2006) Structure 14, 1345–1353
2. M. Gavrilov, et al., (2022) Nature Communications 13:6312

Additional information

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PcrA Helicase (100 μg, 0.5 mg/ml), 000