| SKU | OPTIONS | 价格 | |
|---|---|---|---|
| PcrAH -100 | 100 μg, 0.5 mg/ml | ¥2,289.00 |
- Description
- Additional information
Description
Description
PcrA is an ATP-driven 3′ to 5′ helicase responsible for the unwinding of double-stranded DNA (dsDNA). PcrA binds to and unravels of dsDNA into two strands of single-stranded DNA (ssDNA).
Applications Helicase-dependent DNA isothermal amplification
Source expressed in E. coli
Organism Geobacillus stearothermophilus
Storage -20°C
Storage Buffer
600 mM NaCl, 50 mM Tris pH 7.6, 50% glycerol
10X Reaction Buffer
500 mM Potassium acetate, 200 mM Tris-acetate, 100 mM Magnesium acetate, 1 mg/ml BSA, pH 7.9
Experiment Result
Helicase-dependent DNA isothermal amplification reaction (65 °C, 1 hour) using PcrA helicase for cloning 667 bp and 871 bp DNA fragments. Template of the reaction is 5 ng DNA.
References
1. M. Dittrich, et al., (2006) Structure 14, 1345–1353
2. M. Gavrilov, et al., (2022) Nature Communications 13:6312
Additional information
| OPTIONS | PcrA Helicase, 100 μg, 0.5 mg/ml, 000 |
|---|
