RNase H, E. coli


RNase H is an endoribonuclease that degrades the RNA strand of RNA/DNA hybrid molecules.
SKU: RNHE 分类: ,
RNHE-100 2,500 units (10,000 U/ml) ¥1,594.11
RNHE-200 10,000 units (10,000 U/ml) ¥4,782.44
RNHE-OEM Any Size Please inquire


RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5′-phosphate and 3′-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.

Specific Activity: 625,000 U/mg


  • Removal of poly(A) tails of mRNA hybridized to poly(dT)
  • Removal of mRNA during second strand cDNA synthesis

A recombinant E. coli strain carrying the RNAse H gene (rnh) from E. coli

Supplied in:
20 mM Tris-HCl
100 mM KCl
10 mM MgCl2
0.1 mM EDTA
0.1 mM DTT
50% Glycerol
pH 7.9 @ 25°C

Supplied with:
10x RNAse H Buffer

10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.

Recommended Storage Condition: -20°C



2,500 units (10,000 U/ml), 10,000 units (10,000 U/ml), Any Size

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