Rnase III, E. coli

¥0.00¥6,924.00

Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded RNA, resulting in 12-15 bp dsRNA fragments with 2-base, 3’-overhangs, and 5′ phosphate and 3′ hydroxyl termini.
SKU: RN3E 分类: ,
SKU OPTIONS 价格
RN3E-100 500 units (2,000 U/ml) ¥2,308.00
RN3E-200 2,000 units (2,000 U/ml) ¥6,924.00
RN3E-OEM Any Size Please inquire
B-R310 10X RNase III Reaction Buffer (50 ml) ¥357.00

描述

Description:
Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded RNA, resulting in 12-15 bp dsRNA fragments with 2-base, 3’-overhangs, and 5′ phosphate and 3′ hydroxyl termini.
Source:
Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3′ overhangs.

Applications:

  • Digestion of dsRNA to short fragments
  • RNA structure, processing, and maturation studies

Unit Definition:
1 unit of the Rnase III digests 1 µg of dsRNA in 20 minutes at 37°C

Supplied With:
10X RNase III Reaction Buffer

10X RNase III Reaction Buffer:
500 mM NaCl
100 mM Tris pH 7.9
100 mM MgCl2
10 mM DTT

Storage Buffer:
Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5),100 mM NaCl, 0.1 mM EDTA.

Recommended Storage Condition: -20°C

其他信息

OPTIONS

500 units (2,000 U/ml), 2,000 units (2,000 U/ml), Any Size, 10X RNase III Reaction Buffer (50 ml)

Manual & Protocols

 

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