T7 DNA Ligase

¥594.83¥6,388.41

T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme joins blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
SKU OPTIONS 价格
T7DL-100 1,500 Weiss units (45,000 Weiss U/ml) ¥595.00
T7DL-200 11,250 Weiss units (45,000 Weiss U/ml) ¥2,130.00
T7DL-300 45,000 Weiss units (45,000 Weiss U/ml) ¥6,388.00

描述

Description:
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′-phosphate and a 3′-hydroxyl termini in duplex DNA. The enzyme joins blunt ends and cohesive ends termini as well as repair single-stranded nicks in duplex DNA.

Applications:

  • Joining of Okazaki fragments during replication
  • Completing short-patch DNA synthesis occurring in DNA repair process

Source:
A recombinant E. coli strain carrying the T7 DNA Ligase gene

Supplied in:
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

10x Rapid Ligation Buffer:
660 mM Tris-HCl
100 mM MgCl2
10 mM DTT
10 mM ATP
75% PEG 6000
pH 7.6 @ 25°C

Unit Definition:
One Weiss unit of the enzyme catalyzes the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37°C.  Enzyme activity is assayed in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 0.066 mM ATP, 10 mM DTT, 3.3 µM [32PPi].

One Weiss Unit is approximately equivalent to 67 cohesive end units (CEU). Or 1000 CEU = 15 Weiss Unit.

Specific Activity: 45 Weiss U/µg

Recommended Storage Condition: -20°C

Reference:
Doherty, A. et al. J.Biol. Chem (1996) V.271, No.19, 11083-11089.

其他信息

OPTIONS

1,500 Weiss units (45,000 Weiss U/ml), 11,250 Weiss units (45,000 Weiss U/ml), 45,000 Weiss units (45,000 Weiss U/ml)

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COA ( Certificate of Analysis )

 

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