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Enzymes, Enzymes, Lysozyme, Recombinant Proteins
T4 Lysozyme
Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific…
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Enzymes, Kinases
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules (1).
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DNA/RNA Ligases, Enzymes
T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much…
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Enzymes, Mesophilic DNA Polymerases
T7 DNA Polymerase
T7 DNA Polymerase is a mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is responsible for the rapid and accurate replication of the virus’ genome during its…
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Enzymes, Nucleases
T7 Exonuclease
T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5′ termini liberating 5′ mononucleotides. However, unlike Lambda Exonuclease, the enzyme…
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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR Kits
T7 High Yield In Vitro Transcription Kit
Phage RNA polymerases are widely used for in vitro synthesis of RNA transcripts from DNA templates, which have a double-stranded promoter (at least 19 bases) upstream of the sequence to be…
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Enzymes, Molecular Cloning, Reverse Transcriptase and RNA Polymerases, RT-PCR Kits
T7 RNA Polymerase
T7 RNA polymerase is a DNA-dependent RNA polymerase that catalyzes the formation of RNA in the 5′→ 3′ direction.
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Biochemical Reagents, Biochemical Reagents
TAE (Tris/Acetate/EDTA) Buffer, 10X
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE…
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Enzymes, Mesophilic DNA Polymerases
Terminal deoxynucleotidyl Transferase (TdT)
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.
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